Correct Answer :
C. nonliving content of vacuole.
Cell sap is a non-living fluid manufactured by the cytoplasm of the living plant cell. It consists of water and various substances that are often in the form of a colloidal suspension. The cell sap adds osmotic properties and turgor of the cell, according to the elasticity of plant tissues and organs. It also serves as a receptacle for the water and various substances that participate in cell metabolism and as a place for the deposit of the end products of metabolism.